rabbit polyclonal anti notch3 antibody  (Santa Cruz Biotechnology)

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: NOTCH3 expression in UBC; (A) NOTCH3-positive endothelial cells and NOTCH3-negative (0) tumor cells in pTa UBC (×100); (B) intermediate, focal NOTCH3 (1) staining in high-grade pT1 UBC (×200); (C) intermediate, diffuse NOTCH3 (2) staining in low-grade pT1 UBC (×100); (D) intense, diffuse NOTCH3 (3) staining in pT2 UBC (×200). UBC: Urothelial bladder cancer

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Expressing, Staining

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: Histopathological and clinical characteristics of urothelial bladder cancer patients according to NOTCH3 expression

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Expressing

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: Kaplan–Meier survival curves showing overall survival of 614 bladder cancer patients with negative (0=no staining; 1=weak and/or focal staining) and positive (2=diffuse, intermediate staining; 3=strong, diffuse staining) NOTCH3 expression.

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Staining, Expressing

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: Kaplan–Meier survival curves showing cancer-specific survival of 614 bladder cancer patients with negative (0=no staining; 1=weak and/or focal staining) and positive (2= intermediate, diffuse staining; 3=strong, diffuse staining) NOTCH3 expression.

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Staining, Expressing

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: Kaplan–Meier survival curves showing recurrence-free survival of 614 bladder cancer patients with negative (0=no staining; 1=weak and/or focal staining) and positive (2=intermediate, diffuse staining; 3=strong, diffuse staining) NOTCH3 expression.

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Staining, Expressing

Journal: International Journal of Oncology

Article Title: Regulation of gliomagenesis and stemness through acid sensor ASIC1a

doi: 10.3892/ijo.2021.5262

Figure Lengend Snippet: Notch signaling is negatively associated with ASIC1a in glioblastoma multiforme. (A-C) The glioma cells were transfected with shASIC1a and control followed by assaying protein expression of ASIC1a, active form of Notch1, Notch2, Notch3, Notch4, and Notch target survivin, as well as GSC markers CD133 and ALDH1 by western blotting in (A) A172, (B) U87MG, and (C) PDX-L12 cells. (D-F) Subsequently, the glioma cells (D) A172, (E) U87MG and (F) PDX-L12 were transfected with ASIC1a-FLAG followed by assaying protein expression of ASIC1a, active form of Notch1, Notch2, Notch3, Notch4 and Notch target survivin, as well as GSC markers CD133 and ALDH1 by western blotting. * P<0.05 and ** P<0.01. ASIC1a, acid-sensing ion channel 1a; sh, short hairpin; GSC, glioblastoma stem cells; ALDH1, aldehyde dehydrogenase 1.

Article Snippet: Rabbit polyclonal anti-Notch3 antibody (product code ab60087) was purchased from Abcam.

Techniques: Transfection, Control, Expressing, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: The clinicopathologic significance of Notch3 expression in prostate cancer

doi:

Figure Lengend Snippet: Examples of the expression patterns of Notch3 in cases of prostate cancer (A) No expression; (B) Weak expression; (C) Overexpression (immunohistochemical stain, ×200).

Article Snippet: A rabbit polyclonal anti-Notch3 antibody (1:80, clone sc-515825, Santa Cruz, Biotechnology, CA, USA) was incubated with the sections for 1 h at room temperature.

Techniques: Expressing, Over Expression, Immunohistochemical staining, Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: The clinicopathologic significance of Notch3 expression in prostate cancer

doi:

Figure Lengend Snippet: Correlation between pathological factors and the expression of Notch3 in prostate cancer

Article Snippet: A rabbit polyclonal anti-Notch3 antibody (1:80, clone sc-515825, Santa Cruz, Biotechnology, CA, USA) was incubated with the sections for 1 h at room temperature.

Techniques: Expressing